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Bacteriome identified by next-generation sequencing in saliva, dental plaque, and tumor tissue of patients with oral squamous cell carcinoma

dc.contributorPontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. Medicina Interna
dc.contributorPontificia Universidad Javeriana. Facultad de Medicina. Hospital Universitario San Ignacio
dc.creatorErira, Alveiro
dc.creatorGarcía Robayo, Dabeiba Adriana
dc.creatorChalá, Andrés Ignacio
dc.creatorMoreno Torres, Andrei
dc.creatorMuñoz López, Eliana Elisa
dc.creatorArregui, Angel Cid
dc.creatorTobar, Fabian Tosse
dc.creatorGamboa Jaimes, Fredy Omar
dc.date2022-05-31T16:09:17Z
dc.date2022-05-31T16:09:17Z
dc.date2021-06-07
dc.date.accessioned2025-10-30T13:27:10Z
dc.date.available2025-10-30T13:27:10Z
dc.identifierhttps://www.openmicrobiologyjournal.com/VOLUME/15/PAGE/98/ABSTRACT/
dc.identifier1874-2858
dc.identifierhttp://hdl.handle.net/10554/59944
dc.identifierhttps://doi.org/10.2174/1874285802115010098
dc.identifierinstname:Pontificia Universidad Javeriana
dc.identifierreponame:Repositorio Institucional - Pontificia Universidad Javeriana
dc.identifierrepourl:https://repository.javeriana.edu.co
dc.identifier.urihttp://test.repositoriodigital.com:8080/handle/123456789/181786
dc.description*
dc.descriptionBackground: Oral squamous cell carcinoma (OSCC) is the sixth most common cancer in the world, and the bacterial microbiome has been considered a risk factor that could play an important role in carcinogenesis. Objective: A bacteriome study was performed by next-generation sequencing in dental plaque, saliva, and tumor samples of 10 OSCC patients and compared with bacteriome in dental plaque and saliva of 10 patients without OSCC. Methods: DNA was extracted from all samples and sequenced by Illumina technology MiSeq™. Bioinformatic analyzes were performed for evaluated sequence quality, alpha and beta diversity, bidirectional analysis of variance (p <0.05), and principal component analysis. After establishing bacterial profiles associated with each sample and population, intragroup and intergroup comparisons were carried out. For bacteria identification compatible with eubiosis and dysbiosis processes, a screening was performed based on the frequency of appearance in all patient samples with and without OSCC. Lastly, frequency, average, standard deviation, Chi-square, and Mann Whitney test were calculated. Results: Out of the identified 1,231 bacteria in the populations under study, 45 bacterial species were selected, of which 34 were compatible with eubiosis, and 11 were compatible with dysbiosis. Among the bacteria compatible with eubiosis were species of Lactobacillus and Streptococcus, Chromobacterium violaceum, Enterobacter asburiae, Mycobacterium chubuense, Mycoplasma penetrans, and Brachyspira intermedia. Among the species associated with dysbiosis,  Providencia stuartii, Capnocytophaga canimorsus, Legionella pneumophila, and Mycoplasma hominis were notable. Conclusion: Thirty-four bacterial species may be associated with eubiosis or healthy states and 11 bacterial species could be associated with dysbiosis or pathogenic state, OSCC.
dc.descriptionhttps://orcid.org/ 0000-0003-1509-5631
dc.descriptionhttps://orcid.org/ 0000-0003-0770-9138
dc.descriptionhttps://orcid.org/ 0000-0001-5334-4286
dc.descriptionRevista Internacional - No indexada
dc.descriptionSi
dc.formatPDF
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.relation98
dc.relation110
dc.relationOpen Microbiology Journal
dc.relation15
dc.rightsAtribución-NoComercial 4.0 Internacional
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject*
dc.subjectMicrobiome
dc.subjectEubiosi
dc.subjectDysbiosi
dc.subjectOral squamous cell carcinoma
dc.subjectBacteria
dc.subjectNext-generation sequencing
dc.titleBacteriome identified by next-generation sequencing in saliva, dental plaque, and tumor tissue of patients with oral squamous cell carcinoma
dc.typehttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.typeArtículo de revista
dc.typehttp://purl.org/coar/resource_type/c_6501


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