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Generation of organotypic multicellular spheres by magnetic levitation: model for the study of human hematopoietic stem cells microenvironment

dc.contributorPontificia Universidad Javeriana. Facultad de Medicina. Departamento de Patología
dc.contributorPontificia Universidad Javeriana. Facultad de Medicina. Departamento de Ginecología y Obstetricia
dc.contributorPontificia Universidad Javeriana. Facultad de Medicina. Departamento de Ortopedia y Traumatología
dc.contributorLeal-García, Efraim
dc.creatorMejía-Cruz, Claudia Camila
dc.creatorBarreto Duran, Emilia
dc.creatorPardo-Pérez, María Alejandra
dc.creatorJimenez Achuri, Maria Camila
dc.creatorRincón, Julieth
dc.creatorVanegas, Karen
dc.creatorRodríguez Sarmiento, Jorge Luis
dc.creatorJaramillo-Garcia, Luis Fernando
dc.creatorUlloa, Juan Carlos
dc.creatorMartínez Díaz, Rodolfo
dc.creatorLeal-García, Efraim
dc.creatorPérez-Núñez, Rafael
dc.creatorBarreto, Alfonso
dc.creatorRodriguez, Viviana
dc.date2021-03-19T20:31:44Z
dc.date2021-03-19T20:31:44Z
dc.date2019-02-28
dc.date.accessioned2025-10-30T14:03:29Z
dc.date.available2025-10-30T14:03:29Z
dc.identifierhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457696/
dc.identifier2005-3606 / 2005-5447 (Electrónico)
dc.identifierhttp://hdl.handle.net/10554/53296
dc.identifierhttps://doi.org/10.15283/ijsc18061
dc.identifierinstname:Pontificia Universidad Javeriana
dc.identifierreponame:Repositorio Institucional - Pontificia Universidad Javeriana
dc.identifierrepourl:https://repository.javeriana.edu.co
dc.identifier.urihttp://test.repositoriodigital.com:8080/handle/123456789/193171
dc.descriptionQ4
dc.descriptionQ3
dc.descriptionBackground and Objective: The characteristics of human hematopoietic stem cells are conditioned by the microenvironment of the bone marrow, where they interact with other cell populations, such as mesenchymal stem cells and endothelial cells; however, the study of this microenvironment is complex. The objective of this work was to develop a 3D culture system by magnetic levitation that imitates the microenvironment of human HSC. Methods and Results: Human bone marrow-mesenchymal stem cells, umbilical cord blood-hematopoietic stem cells and a non-tumoral endothelial cell line (CC2811, LonzaⓇ) were used to develop organotypic multicellular spheres by the magnetic levitation method. We obtained viable structures with an average sphericity index greater than 0.6, an average volume of 0.5 mm3 and a percentage of aggregation greater than 70%. Histological studies of the organotypic multicellular spheres used hematoxylin and eosin stains, and an evaluation of vimentin expression by means of immunohistochemistry demonstrated an organized internal structure without picnotic cells and a high expression of vimentin. The functional capacity of human hematopoietic stem cells after organotypic multicellular spheres culture was evaluated by multipotency tests, and it was demonstrated that 3D structures without exogenous Flt3L are autonomous in the maintenance of multipotency of human hematopoietic stem cells. Conclusions: We developed organotypic multicellular spheres from normal human cells that mimic the microenvironment of the human hematopoietic stem cells. These structures are the prototype for the development of complex organoids that allow the further study of the biology of normal human stem cells and their potential in regenerative medicine.
dc.descriptionhttps://orcid.org/0000-0002-9152-5552
dc.descriptionhttps://orcid.org/0000-0003-3075-9854
dc.descriptionhttps://orcid.org/0000-0002-0084-0339
dc.descriptionhttps://orcid.org/0000-0003-1881-9367
dc.descriptionhttps://orcid.org/0000-0003-0157-3639
dc.descriptionN/A
dc.formatPDF
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.relation51
dc.relation62
dc.relationInternational Journal of Stem Cells
dc.relation12
dc.relation1
dc.rightsAtribución-NoComercial 4.0 Internacional
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subjectOrganotypic multicellular spheres
dc.subjectOrganoids
dc.subjectMesenchymal stem cells
dc.subjectHematopoietic stem cells
dc.subjectMicroenvironment
dc.titleGeneration of organotypic multicellular spheres by magnetic levitation: model for the study of human hematopoietic stem cells microenvironment
dc.typehttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.typeArtículo de revista
dc.typehttp://purl.org/coar/resource_type/c_6501


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