Caco-2 cells infected with rotavirus release extracellular vesicles that express markers of apoptotic bodies and exosomes

Bautista López, Diana Marcela; Rodríguez Camacho, Luz Stella; Franco Cortés, Manuel Antonio; Ángel Uribe, Juanita; Barreto Prieto, Alfonso

dc.contributor	Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana
dc.creator	Bautista López, Diana Marcela
dc.creator	Rodríguez Camacho, Luz Stella
dc.creator	Franco Cortés, Manuel Antonio
dc.creator	Ángel Uribe, Juanita
dc.creator	Barreto Prieto, Alfonso
dc.date	2020-02-27T16:24:39Z
dc.date	2020-04-15T13:34:47Z
dc.date	2020-02-27T16:24:39Z
dc.date	2020-04-15T13:34:47Z
dc.date	2015-05-15
dc.date.accessioned	2025-10-30T14:03:48Z
dc.date.available	2025-10-30T14:03:48Z
dc.identifier	https://www.ncbi.nlm.nih.gov/pubmed/25975376
dc.identifier	1355-8145 / 1466-1268 (Electrónico)
dc.identifier	http://hdl.handle.net/10554/47396
dc.identifier	https://doi.org/10.1007/s12192-015-0597-9
dc.identifier.uri	http://test.repositoriodigital.com:8080/handle/123456789/193272
dc.description	Q3
dc.description	Q2
dc.description	Artículo original
dc.description	697-708
dc.description	Previously, we showed that infecting human intestinal epithelial cells (Caco-2) with rotavirus (RV) increases the release of extracellular vesicles (EVs) with an immunomodulatory function that, upon concentration at 100,000×g, present buoyant densities on a sucrose gradient of between 1.10 to 1.18 g/ml (characteristic of exosomes) and higher than 1.24 g/ml (proposed for apoptotic bodies). The effect of cellular death induced by RV on the composition of these EV is unknown. Here, we evaluated exosome (CD63, Hsc70, and AChE) and apoptotic body (histone H3) markers in EVs isolated by differential centrifugation (4000×g, 10,000×g, and 100,000×g) or filtration/ultracentrifugation (100,000×g) protocols. When we infected cells in the presence of caspase inhibitors, Hsc70 and AChE diminished in EVs obtained at 100,000×g, but not in EVs obtained at 4000×g or 10,000×g. In addition, caspase inhibitors decreased CD63 and AChE in vesicles with low and high buoyant densities. Without caspase inhibitors, RV infection increased exosome markers in all of the EVs obtained by differential centrifugation. However, CD63 preferentially localized in the 100,000×g fraction and H3 only increased in EVs concentrated at 100,000×g and with high buoyant densities on a sucrose gradient. Thus, RV infection increases the release of EVs that, upon concentration at 100,000×g, are composed by exosomes and apoptotic bodies, which can partially be separated using sucrose gradients.
dc.format	PDF
dc.format	application/pdf
dc.format	Papel / Electrónico
dc.format	application/pdf
dc.language	spa
dc.rights	Atribución-NoComercial 4.0 Internacional
dc.rights	http://creativecommons.org/licenses/by-nc/4.0/
dc.source	Cell Stress & Chaperones; Vol. 20 Núm. 4 (2015)
dc.subject	Extracellular vesicles
dc.subject	Exosomes
dc.subject	Apoptotic bodies
dc.subject	Apoptotic bodies
dc.subject	Caco-2
dc.subject	Rotavirus
dc.subject	Histone 3
dc.title	Caco-2 cells infected with rotavirus release extracellular vesicles that express markers of apoptotic bodies and exosomes
dc.title	Caco-2 cells infected with rotavirus release extracellular vesicles that express markers of apoptotic bodies and exosomes
dc.type	info:eu-repo/semantics/article
dc.type	http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type	Artículo de revista

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