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Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina

dc.creatorGertiser, María Laura
dc.creatorGiagante, E.
dc.creatorSgattoni, E.
dc.creatorBasabe, N.
dc.creatorRivero, Fernando David
dc.creatorLuján, Hugo Daniel
dc.creatorOcchionero, M.
dc.creatorPannicia, L.
dc.creatorVisciarelli, E.
dc.creatorCostamagna, Sixto
dc.date2010-12-31
dc.date.accessioned2022-11-01T19:35:50Z
dc.date.available2022-11-01T19:35:50Z
dc.identifierhttp://pa.bibdigital.ucc.edu.ar/3442/1/A_Gertiser_Giagante_Sgattoni_Basabe_Rivero_Luj%C3%A1n_Occhionero_Paniccia_Visciarelli_Costamagna.pdf
dc.identifier.urihttp://test.repositoriodigital.com:8080/handle/123456789/43982
dc.descriptionIntroduction. Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures. Methods. New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall. Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones. Results. The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality. Conclusions. The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.
dc.descriptionFil: Gertiser, María Laura. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.descriptionFil: Giagante, E. MEGAVISIÓN. Centro Privado de Oftalmología; Argentina
dc.descriptionFil: Sgattoni, E. MEGAVISIÓN. Centro Privado de Oftalmología; Argentina
dc.descriptionFil: Basabe, N. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.descriptionFil: Rivero, Fernando David. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
dc.descriptionFil: Luján, Hugo Daniel. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
dc.descriptionFil: Occhionero, M. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.descriptionFil: Pannicia, L. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.descriptionFil: Visciarelli, E. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.descriptionFil: Costamagna, Sixto. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.formatapplication/pdf
dc.languagespa
dc.publisherAsociación Argentina de Microbiología
dc.relationhttp://pa.bibdigital.ucc.edu.ar/3442/
dc.relationhttps://www.redalyc.org/articulo.oa?id=213014892011
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.sourceGertiser, María Laura ORCID: https://orcid.org/0000-0001-6820-8690 <https://orcid.org/0000-0001-6820-8690>, Giagante, E., Sgattoni, E., Basabe, N., Rivero, Fernando David, Luján, Hugo Daniel ORCID: https://orcid.org/0000-0002-3797-8315 <https://orcid.org/0000-0002-3797-8315>, Occhionero, M., Pannicia, L., Visciarelli, E. and Costamagna, Sixto ORCID: https://orcid.org/0000-0001-8358-6155 <https://orcid.org/0000-0001-8358-6155> (2010) Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina. Revista Argentina de Microbiología, 42 (2). pp. 122-125. ISSN 0325-7541
dc.subjectQD Química
dc.titleQueratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/acceptedVersion


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