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Determinación del tamaño del genoma de <i>Gmelina arborea</i> Roxb. utilizando citometría de flujo

dc.contributor.authorYaya Lancheros, Mary Luzspa
dc.contributor.authorTerán, Wilsonspa
dc.date.accessioned2020-01-01 00:00:00
dc.date.accessioned2023-09-19T21:10:23Z
dc.date.available2020-01-01 00:00:00
dc.date.available2023-09-19T21:10:23Z
dc.date.issued2020-01-01
dc.identifier.issn0120-0739
dc.identifier.urihttp://test.repositoriodigital.com:8080/handle/123456789/44472
dc.description.abstractLa melina es una especie forestal con una alta calidad maderable y de importancia económica para diferentes países tropicales como Colombia. No obstante, existe poca información acerca de su genética, lo cual es pertinente en programas de mejoramiento. Con el fin de determinar el tamaño del genoma de Gmelina arborea (valor C), se obtuvieron suspensiones nucleares a partir de hojas, las cuales se analizaron mediante citometría de flujo. Para la obtención de suspensiones acuosas de núcleos, se evaluaron cuatro buffers de extracción, de los cuales, el buffer LB01 produjo poblaciones nucleares intactas y con mayor homogeneidad. Se analizaron tres especies vegetales como posibles estándares para la determinación del valor C; utilizando tomate como estándar referente, se obtuvo un valor C para melina de 467 Mpb. Este es el primer reporte relacionado con la estimación del valor C en melina, por lo que se genera así información importante para incrementar el conocimiento relacionado con esta especie.spa
dc.description.abstractMelina, also known as white teak, is a species of high timber quality and economic importance for different tropical countries like Colombia. However, information about its genetics is scarce, which is relevant for breeding programs. In order to determine the genome size of Gmelina arborea (C value), nuclei suspensions were obtained from leaves, and analyzed using flow cytometry. To obtain aqueous nuclei suspensions, four extraction buffers were evaluated, LB01 buffer produced intact populations with greater homogeneity. Three species were analyzed as possible reference standards for the determination of the C value; using tomato as a reference species, a C value for melina of 467 Mpb was obtained. This is the first report related to the estimation of the C value in Gmelina arborea, generating important information to increase knowledge related to this specieseng
dc.format.mimetypeapplication/pdfspa
dc.format.mimetypetext/xmlspa
dc.language.isospaspa
dc.publisherUniversidad Distrital Francisco José de Caldasspa
dc.rightsColombia forestal - 2020spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourcehttps://revistas.udistrital.edu.co/index.php/colfor/article/view/14931spa
dc.subjectC-valueeng
dc.subjectfluorescenceeng
dc.subjectnucleo isolationeng
dc.subjectaislamiento de núcleosspa
dc.subjectfluorescenciaspa
dc.subjectvalor Cspa
dc.titleDeterminación del tamaño del genoma de <i>Gmelina arborea</i> Roxb. utilizando citometría de flujospa
dc.typeArtículo de revistaspa
dc.identifier.doi10.14483/2256201X.14931
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
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dc.type.localJournal articleeng
dc.title.translatedGmelina arborea Roxb. genome-size determination using flow cytometryeng
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.relation.referencesAliyu, O. (2012). Development of the flow cytometric protocol for ploidy analysis and determination of relative nuclear DNA content in cashew (Anacardium occidentale Linn.). American Journal of Biochemistry and Molecular Biology, 2(4), 200–215. https://doi.org/10.3923/ajbmb.2012.200.215.spa
dc.relation.referencesAtri, C. y Banga, S. (2014). A protocol for flow cytometric determination of expected chromosome number of Brassica juncea L. introgression lines. Journal of Oilseed Brassica, 5(2), 170–174.spa
dc.relation.referencesBennett, M. D. (2005). Nuclear DNA Amounts in Angiosperms: Progress, Problems and Prospects. Annals of Botany, 95(1), 45–90. https://doi.org/10.1093/aob/mci003spa
dc.relation.referencesBourge, M., Brown, S. C. y Siljak-yakovlev. S. (2018). Flow cytometry as tool in plant sciences, with emphasis on genome size and ploidy level assessment. Genetics & Applications, 2(2), 1-12. https://doi.org/10.31383/ga.vol2iss2pp1-12.spa
dc.relation.referencesDoležel, J. (2005). Plant DNA Flow Cytometry and Estimation of Nuclear Genome Size. Annals of Botany, 95(1), 99–110. https://doi.org/10.1093/aob/mci005.spa
dc.relation.referencesDoležel, J., Binarová, P. y Lucretti, S. (1989). Analysis of Nuclear DNA content in plant cells by Flow cytometry. Biologia Plantarum, 31(2), 113–120. https://doi.org/10.1007/BF02907241spa
dc.relation.referencesDoležel, J., Doleželová, M. y Novák, F. J. (1994). Flow cytometric estimation of nuclear DNA amount in diploid bananas (Musa acuminata and M. balbisiana). Biologia Plantarum, 36(3), 351–357. https://doi.org/10.1007/BF02920930.spa
dc.relation.referencesDoležel, J. y Greilhuber, J. (2010). Nuclear genome size: Are we getting closer? Cytometry, 77(7), 635–42. https://doi.org/10.1002/cyto.a.20915.spa
dc.relation.referencesDoležel, J., Greilhuber, J. y Suda. J. (2007). Estimation of nuclear DNA content in plants using flow cytometry. Nature Protocols, 2(9), 2233–2244. https://doi.org/10.1038/nprot.2007.310.spa
dc.relation.referencesDvorak, W. S. (2004). World view of Gmelina arborea: opportunities and challenges. New Forests, 28(2/3):111–126. https://doi.org/10.1023/B:NEFO.0000040940.32574.22.spa
dc.relation.referencesGonzález-Rojas, M., Murillo-Cruz, R. y Ávila-Arias, C. (2016). Crecimiento inicial de Gmelina arborea Roxb. aplicando diferentes tratamientos de fertilización en el Pacífico Sur de Costa Rica. Revista Forestal Mesoamericana Kurú, 13(33), 29-35. https://doi.org/10.18845/rfmk.v13i33.2575.spa
dc.relation.referencesGreilhuber, J. (2008). Cytochemistry and C-values: The Less-well-known World of Nuclear DNA Amounts. Annals of Botany, 101(6):791–804. https://doi.org/10.1093/aob/mcm250spa
dc.relation.referencesHunt, SE., Mclaren, W., Gil, L., Thormann, A., Schuilenburg, H., Sheppard, D., Parton, A., Armean, S., Trevanion, S., Flicek, P. y Cunningham, F. (2018). Ensembl variation resources. Database, 2018. https://doi.org/10.1093/database/bay119.spa
dc.relation.referencesJedrzejczyk, I. y Sliwinska, E. (2010). Leaves and seeds as materials for flow cytometric estimation of the genome size of 11 rosaceae woody species containing DNA-staining inhibitors. Journal of Botany, 2010, 1–9. http://dx.doi.org/10.1155/2010/930895.spa
dc.relation.referencesLeitch, I. J. (1998). Phylogenetic Analysis of DNA C-values provides Evidence for a Small Ancestral Genome Size in Flowering Plants. Annals of Botany, 82, 85–94. https://doi.org/10.1006/anbo.1998.0783spa
dc.relation.referencesLeitch, I. J., Johnston, E., Pellicer, J., Hidalgo, O. y Bennett, M. D. (2019). Plant DNA C-values database (release 7.0). Recuperado de https://cvalues.science.kew.org/.spa
dc.relation.referencesLoureiro, J., Rodriguez, E., Doležel, J. y Santos, C. (2006). Comparison of Four Nuclear Isolation Buffers for Plant DNA Flow Cytometry. Annals of Botany, 98(3), 679–689. https://doi.org/10.1093/aob/mcl141spa
dc.relation.referencesLoureiro, J., Rodriguez, E., Doležel, J y Santos C. (2007). Two new nuclear isolation buffers for plant DNA flow cytometry: A test with 37 species. Annals of Botany, 100, 875-888. https://doi.org/10.1093/aob/mcm152spa
dc.relation.referencesLutz, K. A., Wang, W., Zdepski, A. y Michael, T. P. (2011). Isolation and analysis of high quality nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing. BMC Biotechnology, 11(54), 1-9. https://doi.org/10.1186/1472-6750-11-54spa
dc.relation.referencesMoya, R. y Tomazello Fo, M. (2006). Variation in the wood anatomical structure of Gmelina arborea (Verbenaceae) trees at different ecological conditions in Costa Rica. Revista de Biología Tropical, 56(2), 689-704.spa
dc.relation.referencesOhri, D. y Kumar, A. (1986). Nuclear DNA Amounts in Some Tropical Hardwoods. Caryologia, 39(3–4), 303–307. https://doi.org/10.1080/00087114.1986.10797792spa
dc.relation.referencesOtto, F. (1990). DAPI Staining of Fixed Cells for High-Resolution Flow Cytometry of Nuclear DNA. Methods in Cell Biology, 33, 105-110. https://doi.org/10.1016/S0091-679X(08)60516-6spa
dc.relation.referencesPellicer, J., Hidalgo, O., Dodsworth, S. y Leitch, I. J. (2018). Genome Size Diversity and Its Impact on the Evolution of Land Plants. Genes (Basel), 9(2), 1-14. https://doi.org/10.3390/genes9020088spa
dc.relation.referencesProta. (2016). PROTA4U, Plant Resources of Tropical Africa: web database. Recuperado de https://www.prota4u.org/database/.spa
dc.relation.referencesRojas, A., Moreno, L., Melgarejo, L. M. y Rodríguez, M. (2012). Physiological response of gmelina (Gmelina arborea Roxb.) to hydric conditions of the Colombian Caribbean. Agronomia Colombiana, 30(1), 52-58.spa
dc.relation.referencesSliwinska, E. (2018). Flow cytometry – a modern method for exploring genome size and nuclear DNA synthesis in horticultural and medicinal plant species. Folia Horticulturae, 30(1), 103-128. https://doi.org/10.2478/fhort-2018-0011.spa
dc.relation.referencesSrisawat, T., Pattanapanyasat, K. y Doležel, J. (2012). Flow cytometric classification of oil palm cultivars. African Journal of Biotechnology, 11(16), 3714–24.spa
dc.relation.referencesTenorio, C., Moya, R., Arias-Aguilar, D. y Briceño-Elizondo, E. (2016). Biomass yield and energy potential of short-rotation energy plantations of Gmelina arborea one-year-old in Costa Rica. Industrial Crops and Products, 82, 63–73. https://doi.org/10.1016/j.indcrop.2015.12.005.spa
dc.relation.referencesUrrego, J. B. (2004). Growth potential of Gmelina arborea at 3 years of age in Colombia. New Forests, 28(2/3), 269–276. https://doi.org/10.1023/B:NEFO.0000040953.91091.69spa
dc.relation.referencesVrána, J., Cápal, P., Bednářová, M y Doležel, J. (2014). Flow Cytometry in Plant Research: A Success Story. En: P. Nick y Z. Opatrny (eds). Applied Plant Cell Biology: Cellular tools and approaches for plant biotechnology (pp 395-430). Berlin: Springer-Verlag Berlin Heidelberg Press.spa
dc.relation.referencesWang, J., Liu, J. y Kang, M. (2015). Quantitative testing of the methodology for genome size estimation in plants using flow cytometry: a case study of the Primulina genus. Frontiers in Plant Science, 6(354), 1-13. https://doi.org/10.3389/fpls.2015.00354.spa
dc.relation.referencesZhou, M., Xu, C., Shen, L., Xiang, W. y Tang, D. (2017). Evolution of genome sizes in Chinese Bambusoideae (Poaceae) in relation to karyotype. Trees, 31(1), 41–48. https://doi.org/10.1007/s00468-016-1453-yspa
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dc.relation.citationvolume23spa
dc.relation.citationissue1spa
dc.relation.citationeditionNúm. 1 , Año 2020 : Enero-Juniospa
dc.relation.ispartofjournalColombia forestalspa
dc.identifier.eissn2256-201X
dc.identifier.urlhttps://doi.org/10.14483/2256201X.14931
dc.relation.citationstartpage68
dc.relation.citationendpage78
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Colombia forestal - 2020
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