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Development and evaluation of an alternative culture medium for mass cultivation of Azospirillum brasilense C16 using sequential statistical designs

Desarrollo y evaluación de un medio de cultivo alternativo para la multiplicación de Azospirillum brasilense C16 mediante diseños estadísticos secuenciados

dc.creatorMoreno-Galván, Andrés
dc.creatorRojas-Tapias, Daniel
dc.creatorBonilla, Ruth
dc.date2013-01-17
dc.date.accessioned2020-08-04T20:35:33Z
dc.date.available2020-08-04T20:35:33Z
dc.identifierhttp://revista.corpoica.org.co/index.php/revista/article/view/256
dc.identifier10.21930/rcta.vol13_num2_art:256
dc.identifier.urihttp://test.repositoriodigital.com:8080/handle/123456789/4537
dc.descriptionFor mass plant growth-promoting inoculant production, a high-yield culture medium is fundamental. Sequential application of statistical designs was used to optimize Azospirillum brasilense C16 biomass production. Six nutritional (glycerol, glutamate, mannitol, citric acid, yeast extract and K2HPO4 3H2O) and three mineral sources (MgSO4 7H2O, FeCl3 and NaCl) were evaluated using five statistical experiments Placket-Burman, factorial design, steepest ascent, response surface analysis, and mineral screening. The optimum medium composition (g L-1) was as follows: 28.33 glutamate, 2.92 yeast extract, 1.34 K2HPO4 3H2O, 0.5 MgSO4 7H2O and 0.02 FeCl3. After 24 hours of incubation, protein (32.04 μg) and dry biomass (1.51 g L-1) were 1.72 and 1.68 times higher than in conventional growth medium.  en-US
dc.descriptionPara la producción masiva de inoculantes basados en bacterias promotoras de crecimiento vegetal (PGPR), es fundamental un medio de cultivo de alto rendimiento. La aplicación secuenciada de diseños estadísticos fue usada para optimizar la producción de biomasa de Azospirillum brasilense C16, seis fuentes nutricionales (glicerol, glutamato, manitol, acido cítrico, extracto de levadura y K2HPO4 3H2O) y tres fuentes minerales (MgSO4 7H2O, FeCl3 y NaCl) fueron evaluadas mediante cinco experimentos estadísticos - Placket-Burman, factorial fraccionado, diseño de paso ascendente, análisis de superficie de respuesta y screening mineral, para tal efecto. La composición optimizada del medio (g L-1) fue: 28,33 glutamato, 2,92 extracto de levadura, 1,34 K2HPO4 3H2O, 0,5 MgSO4 7H2O y 0,02 FeCl3, la cual luego de 24 h de incubación permitió producir una cantidad de proteína (32,04 μg) y biomasa seca (1,51 g L-1) del 1,72 y 1,68 veces más alta, respectivamente, en relación al medio de cultivo convencional.   es-ES
dc.formatapplication/pdf
dc.languagespa
dc.publisherCorporación Colombiana de Investigación Agropecuaria (Agrosavia)es-ES
dc.relationhttp://revista.corpoica.org.co/index.php/revista/article/view/256/259
dc.sourceCiencia y Tecnología Agropecuaria; Vol. 13 No. 2 (2012); 201-206en-US
dc.sourceCiencia & Tecnología Agropecuaria; Vol. 13 Núm. 2 (2012); 201-206es-ES
dc.sourcerevista Corpoica Ciência e Tecnologia Agropecuária; v. 13 n. 2 (2012); 201-206pt-BR
dc.source2500-5308
dc.source0122-8706
dc.source10.21930/rcta.vol13-num2
dc.subjectculture medium optimizationen-US
dc.subjectfactorial designsen-US
dc.subjectculture medium designen-US
dc.subjectbioinoculant productionen-US
dc.subjectOptimización De Medio De Cultivoes-ES
dc.subjectDiseños Factorialeses-ES
dc.subjectDiseño De Medio De Cultivoes-ES
dc.subjectProducción De Bioinoculanteses-ES
dc.titleDevelopment and evaluation of an alternative culture medium for mass cultivation of Azospirillum brasilense C16 using sequential statistical designsen-US
dc.titleDesarrollo y evaluación de un medio de cultivo alternativo para la multiplicación de Azospirillum brasilense C16 mediante diseños estadísticos secuenciadoses-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
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