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Evaluation of sucrose and GA3 in an in vitro shoot culture of Alpinia purpurata (Zingiberaceae): Sucrose and GA3 on Alpinia purpurata in vitro shoots.

Evaluación de sacarosa y GA3 en un cultivo in vitro de brotes de Alpinia purpurata (Zingiberaceae)

dc.creatorSuarez Padrón, Isidro Elías
dc.creatorPérez Meza, Pablo Miguel
dc.creatorLopez Diaz, Claudia Marcela
dc.date2020-05-04
dc.date.accessioned2020-08-04T20:36:44Z
dc.date.available2020-08-04T20:36:44Z
dc.identifierhttp://revista.corpoica.org.co/index.php/revista/article/view/1193
dc.identifier10.21930/rcta.vol21_num2_art:1193
dc.identifier.urihttp://test.repositoriodigital.com:8080/handle/123456789/4682
dc.descriptionAlpinia purpurata is an ornamental plant species native to the Asia-Pacific with commercial potential due to its red inflorescence adapted to the northern Colombian coast; however, the lack of propagation protocols hinders commercial cultivation. To adjust a micropropagation protocol for clonal plant material production, the aim of this study was to evaluate the effect of sucrose and gibberellic acid (GA3) in the culture medium on the number of new shoots per explant, shoot length, root number, root length and number of leaves. Explants were cultured in semisolid MS (Murashige & Skoog) medium with sucrose (30, 45 and 60 mg/L) and GA3 (0.0; 0.25; 0.5; 0.75 and 1.0 mg/L). A factorial experimental design with two factors was employed, with 15 treatments, 10 replicates per treatment and 150 experimental units distributed with a completely randomized design. Cultures were stored five weeks at 25 °C with a 12 h photoperiod (40 μmol m-2 s-1). Anova and Tukey’s test allowed detecting the statistical effects of sucrose and GA3 supply for all variables. An increase in sucrose concentration resulted in a higher number of shoots, while an increase in GA3 induced longer shoots. Root length and leaf number decreased as GA3 and sucrose increased.en-US
dc.descriptionAlpinia purpurata es una planta ornamental nativa de las islas del Pacífico asiático con potencial por su inflorescencia roja, adaptada a las condiciones de la costa Atlántica colombiana; sin embargo, la falta de protocolos de propagación, evita su cultivo comercialmente. Con el interés ajustar un protocolo de micropropagación para la producción clonal de material de siembra, el presente estudio tuvo como objetivo, evaluar el efecto de la sacarosa y el ácido giberélico (GA3) en el medio de cultivo, sobre el número de nuevos brotes por explante, longitud de brotes, número de raíces, longitud de raíces y número de hojas. Los explantes fueron cultivados en medio semisólido ms (Murashige & Skoog, 1962) suplido con sacarosa (30, 45 y 60 mg/L) y combinados con GA3 (0,0; 0,25; 0,5; 0,75 y 1,0 mg/L). Se empleó un arreglo factorial con dos factores, 15 tratamientos y 10 repeticiones por tratamiento, para un total de 150 unidades experimentales distribuidas en un diseño completamente al azar. Los cultivos fueron almacenados cinco semanas a 25 °C con 12 h de fotoperíodo (40 μmol m-2 s-1). El Anova y la prueba de Tukey permitieron detectar que la sacarosa y GA3 afectaron significativamente todas las variables: el incremento en la concentración de sacarosa indujo mayor número de brotes, y los brotes en presencia de GA3 tuvieron una mayor longitud. Se observó una tendencia a disminuir la longitud de las raíces y el número de hojas en la medida en que se aumentó la dosis de sacarosa y GA3.es-ES
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.formattext/xml
dc.formattext/xml
dc.languagespa
dc.languageeng
dc.publisherCorporación Colombiana de Investigación Agropecuaria (Agrosavia)es-ES
dc.relationhttp://revista.corpoica.org.co/index.php/revista/article/view/1193/624
dc.relationhttp://revista.corpoica.org.co/index.php/revista/article/view/1193/640
dc.relationhttp://revista.corpoica.org.co/index.php/revista/article/view/1193/661
dc.relationhttp://revista.corpoica.org.co/index.php/revista/article/view/1193/662
dc.rightsDerechos de autor 2020 Ciencia & Tecnología Agropecuariaes-ES
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0/deed.eses-ES
dc.sourceCiencia y Tecnología Agropecuaria; Vol. 21 No. 2 (2020): Ciencia &Tecnología Agropecuariaen-US
dc.sourceCiencia & Tecnología Agropecuaria; Vol. 21 Núm. 2 (2020): Ciencia &Tecnología Agropecuariaes-ES
dc.sourcerevista Corpoica Ciência e Tecnologia Agropecuária; v. 21 n. 2 (2020): Ciencia &Tecnología Agropecuariapt-BR
dc.source2500-5308
dc.source0122-8706
dc.source10.21930/rcta.vol21-num2
dc.subjectInflorescenceen-US
dc.subjectmicropropagationen-US
dc.subjectornamental plantsen-US
dc.subjectplant growth substancesen-US
dc.subjectred gingeren-US
dc.subjectInflorescenciaes-ES
dc.subjectjengibre rojoes-ES
dc.subjectmicropropagaciónes-ES
dc.subjectplantas ornamentaleses-ES
dc.subjectsustancias de crecimiento vegetales-ES
dc.titleEvaluation of sucrose and GA3 in an in vitro shoot culture of Alpinia purpurata (Zingiberaceae): Sucrose and GA3 on Alpinia purpurata in vitro shoots.en-US
dc.titleEvaluación de sacarosa y GA3 en un cultivo in vitro de brotes de Alpinia purpurata (Zingiberaceae)es-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
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