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Response Surface Methodology (RSM) for analysing culture conditions of Acidocella facilis strain USBA-GBX-505 and Partial Purification and Biochemical Characterization of Lipase 505 LIP

dc.contributorPontificia Universidad Javeriana (Project ID 4269) and Colciencias – Sena. (Project 6570-392-199990).
dc.creatorBernal, Luisa Fernanda; Colombian Centre for Genomics and Bioinformatics of Extreme Environments - GeBiX. Carrera 5 No. 66A-34, 110231, Bogotá D.C. - Colombia. Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, POB 56710, Bogotá D.C. - Colombia.
dc.creatorLopez, Gina; Colombian Centre for Genomics and Bioinformatics of Extreme Environments - GeBiX. Carrera 5 No. 66A-34, 110231, Bogotá D.C. - Colombia. Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, POB 56710, Bogotá D.C. - Colombia.
dc.creatorRuiz, Monica; Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, POB 56710, Bogotá D.C. - Colombia.
dc.creatorVera-Bravo, Ricardo; Química de Macromoléculas. Facultad de Ciencias, Pontificia Universidad Javeriana. POB 56710, Bogotá D.C. - Colombia
dc.creatorReyes, Alejandro; Química de Macromoléculas. Facultad de Ciencias, Pontificia Universidad Javeriana. POB 56710, Bogotá D.C. - Colombia
dc.creatorBaena, Sandra; Colombian Centre for Genomics and Bioinformatics of Extreme Environments - GeBiX. Carrera 5 No. 66A-34, 110231, Bogotá D.C. - Colombia. Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, POB 56710, Bogotá D.C. - Colombia.
dc.date2018-02-24T16:00:11Z
dc.date2020-04-15T18:07:59Z
dc.date2018-02-24T16:00:11Z
dc.date2020-04-15T18:07:59Z
dc.date2017-02-20
dc.date.accessioned2025-10-30T14:03:36Z
dc.date.available2025-10-30T14:03:36Z
dc.identifierhttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/15356
dc.identifier10.11144/Javeriana.SC22-1.rsmr
dc.identifier2027-1352
dc.identifier0122-7483
dc.identifierhttp://hdl.handle.net/10554/31581
dc.identifier.urihttp://test.repositoriodigital.com:8080/handle/123456789/193213
dc.descriptionUsing Response Surface Methodology (RSM) we evaluated the culture conditions (nitrogen source, carbon source, pH and agitation rate) that increase the biomass of Acidocella facilis strain USBA-GBX-505 and therefore enhance the production of its lipolytic enzyme, 505 LIP. RSM results revealed that yeast extract and agitation were key culture factors that increased the growth-associated lipolytic activity by 4.5-fold (from 0.13 U.mg-1 to 0.6 U.mg-1). The 505 LIP lipase was partially purified using size-exclusion chromatography and ion-exchange chromatography. Its molecular weight was >77 kDa. The enzyme shows its optimum catalytic activity at 55 °C and pH 7.5. EDTA, PMSF, 1-butanol and DMSO inhibited enzymatic activity, whereas Tween 20, acetone, glycerol and methanol increased it. Metallic ions are not required for the activity of 505 LIP, and even have an inhibitory effect on the enzyme. This study shows the potential use of A. facilis strain USBA-GBX-505 for the production of a newly identified lipolytic enzyme, 505 LIP, which is stable at moderate temperatures and in the presence of organic solvents. These are important characteristics for the synthesis of many useful products.
dc.formatPDF
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Javeriana
dc.relationhttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/15356/14569
dc.relationhttp://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15356/9772
dc.relationUniversitas Scientiarum; Vol 22, No 1 (2017); 45-70
dc.relationUniversitas Scientiarum; Vol 22, No 1 (2017); 45-70
dc.relationUniversitas Scientiarum; Vol 22, No 1 (2017); 45-70
dc.rightsCopyright (c) 2017 Universitas Scientiarum
dc.rightsCreative Commons Attribution 4.0 International Public License
dc.rightsAtribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subjectMicrobiology
dc.subjectlipases; acidophiles; Acidocella facilis; stability in solvents; Plackett-Burman design; central composite design.
dc.subjectEnzymes
dc.titleResponse Surface Methodology (RSM) for analysing culture conditions of Acidocella facilis strain USBA-GBX-505 and Partial Purification and Biochemical Characterization of Lipase 505 LIP
dc.typehttp://purl.org/coar/version/c_970fb48d4fbd8a85
dc.typeArtículo de revista
dc.typehttp://purl.org/coar/resource_type/c_6501
dc.typeinfo:eu-repo/semantics/article
dc.coverageColombia
dc.coveragenull
dc.coveragenull


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